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Role of Thrombopoietin in Rescuing Vascular Niche of ITP Patients

Y. Jiao, X. Zeng, Z. Li, Y. Zhang, J. Ye

Southern Hospital, Southern Medical University, Department of Hematology, Guang zhou, China

Abstract Number: PB1960

Meeting: ISTH 2020 Congress

Theme: Vascular Biology » Blood Cells and Vessel Wall

Background: The vascular niche of immune thrombocytopenia (ITP) is proven to be damaged.
It is presented as the impaired functions of migration and tube-formation in bone marrow endothelial progenitor cells (BM-EPCs). Our previous studies shown that thrombopoietin (TPO) has a protective effect on human endothelial cells.

Aims: This study aims to investigate whether TPO has a protective effect in ITP-EPCs, and to explore its potential mechanism.

Methods: BM-EPCs were isolated from ITP patients, and divided into two groups: TPO-treated group and TPO-free (control) group. CCK8 was used to explore the proliferative effect of TPO on BM-EPCs. BM-EPCs was identified by flow cytometry with co-expression of mouse anti-humanCD133, mouse anti-human CD34 and mouse anti-human vascular endothelial growth factor receptor (CD309) monoclonal antibodies. Moreover, the number of EPCs was evaluated with DiL-Ac-LDL uptake and FITC-UEA -I binding assay. The BM-EPCs functions were examed by tube-formation and migration experiments.

Results: The EPCs isolated from ITP patient in TPO-treated group exhibited longer fusiform and elliptical shape after 5 days and cobblestone-like morphology after 7-10 days culture. The result of CCK-8 showed TPO promoted proliferation of EPCs and the optional drug concentration is 100 ng/ml. Much higher co-expression of CD34/CD133/CD309 in TPO-treated group was detected by flow cytometry. The number of DiL-Ac-LDL (red) and FITC-UEA -I (green) double-stained cells in the TPO group was significantly higher than that of control group (n=10, p<0.05). The tube formation (n=10, p< 0.05) and migration capacity (n=8, p< 0.05) were significantly enhanced in TPO-treated group.

Conclusions: Our studies indicates that TPO exerts protective effect of BM-EPCs in ITP patients, which may rescue megakaryopoiesis and reduce hemorrhage in these cases.

To cite this abstract in AMA style:

Jiao Y, Zeng X, Li Z, Zhang Y, Ye J. Role of Thrombopoietin in Rescuing Vascular Niche of ITP Patients [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/role-of-thrombopoietin-in-rescuing-vascular-niche-of-itp-patients/. Accessed September 27, 2023.

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