Background: It has been shown that hormones of neurohypophysis  and hypothalamus can have effects on blood clotting when interacting with specific receptors of blood cells and endothelium. Along with the neuropeptides themselves, their fragments formed during enzymatic degradation can have independent biological activity.

Aims: In this study was to compare the effect of small regulatory peptides, which are fragments of neurohormones, in  vitro experiments on the aggregation of red blood cells and platelets under the action of epinephrine.

Methods: C – terminal fragments of vasopressin, oxytocin, luliberin and the hormone thyroliberin  was used
Pro-Arg-Gly-NH2. – vasopressin fragment (P1)
Pro-Leu-Gly-NH2-oxytocin fragment, (P2)
Arg-Pro-Gly-NH2-lyuliberin fragment (P3)
Glu-His – Pro – NH2-tyroliberin (P4)
Peptides are added in a concentration range of 10-1 – 10-10M or an equal volume of saline added, or  to a pool of washed platelets, or erythrocytes and the measured change in  aggregation  by the action of epinephrine 0.02 mmol / l on a aggregometer. The results were treated statistically.

Results: It has been shown that small regulatory peptides, which are products of the proteolysis of neuropeptides, have a different action on the aggregative state of blood cells stimulated by adrenaline. It was found that PI causes a significant increase in the aggregation of both red blood cells and platelets, (p<0.001), whereas P2 inhibited only erythrocyte aggregation, without changing platelet aggregation. P3 had virtually no effect on platelet aggregation. P4 increased both platelet aggregation and erythrocyte aggregation (p<0.001).

Conclusions: The effect of the studied tripeptides on hemostasis can be carried out through specific receptors on both blood cells and endothelium. Given their high efficiency, a  differentiated approach to the therapy of thrombotic complication is required.

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ISTH Congress Abstracts - https://abstracts.isth.org/abstract/short-peptides-in-the-regulation-blood-cells-aggregation-in-thrombogenesis/