Abstract Number: PB1611
Meeting: ISTH 2020 Congress
Background: Caffeic acid (CFA) is a commonly used medicine in China for treatment of various causes of thrombocytopenia and leukocytopenia, but the mechanism is unclear.
Aims: To investigate the effect of CFA on umbilical cord blood hematopoietic stem cells (UCB-HSCs) in vitro, and explore the effect of CFA on cell expansion and differentiation in various tissues in vivo.
Methods: CD34+ UCB-HSCs were cultured with or without CFA for 6 days, total cell number and proportion of CD34+ cells were detected. Differentiation ability was analyzed by colony formation assay and megakaryocytes inducing assay. Xenotransplantation of CFA-treated HSC to detect Hematopoietic reconstitution capacity. Additionally, to determine BrdU uptake rate in bone marrow and other tissues, CD-1 mice were administered with CFA or vehicle for 14 days and BrdU injection started from day 7. Finally, mRNA and protein expression levels of c-fos, cyclinD1 and CKD4 were assessed.
Results: In HSC expansion tests, after 6-day culture of CD34+ cells, the total number of cells in CFA group(8.45±4.89*105) was significantly higher than that in control group(6.23±4.24*105; P=0.006). In HSC colony formation assay and megakaryocytes inducing assay, there were no significant difference between CFA groups and control. In vivo, the uptake capacity of BrdU by bone marrow mononuclear cells but not any other tissue in CFA group was significantly higher than that in control group, which indicating the tissue specificity of CFA. Elevated mRNA and protein expression level of c-fos, c-jun, cyclinD1 and CKD4 were detected in 15 to 30 minutes after cultured with 10µg/mL CFA in CD34+ cells.
Conclusions: In conclusion, CFA can promote the proliferation of bone marrow and umbilical cord HSCs by stimulating expression of cfos and speeding up cell cycle process through cyclinD1 and CKD4, which further revealed the pharmacological effects of CFA.
To cite this abstract in AMA style:Wang R, Liu Y, Liu X, Liu Y, Wang Y, Hou M. Short-term Application of Caffeic Acid Specifically Promotes Expansion of Hematopoietic Stem Cells [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/short-term-application-of-caffeic-acid-specifically-promotes-expansion-of-hematopoietic-stem-cells/. Accessed February 20, 2024.
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