Abstract Number: PB1001
Meeting: ISTH 2021 Congress
Background: Platelet-rich plasma (PRP) has recently been used in human and veterinary regenerative medicine due to its abundant content of growth factors (GFs) and cytokines that can affect tissue regeneration, wound healing, angiogenesis, and cell proliferation. However, no standardized method of PRP preparation has been established. Moreover, there have been no studies on the effects of the currently used methods on platelet function which further affects the optimal use of PRP in the regenerative field.
Aims: Therefore, we determined to optimize the PRP preparation to achieve the highest recovery of platelets with the characterization of relevant platelet function and GF release using canine platelets.
Methods: Whole blood from healthy dogs was centrifuged at different run conditions (800, 1200, 1500, 2000, and 3600 rpm) for 10 min single-spin or 5 min dual-spin to obtain PRP. Commercial PRP preparation kit was used to compare its efficiency. The platelet recovery rate of each PRP was determined by CBC. Agonist-induced platelet aggregation and Platelet-Derived Growth Factor-BB (PDGF-BB) release were measured to determine the function of platelets prepared from each PRP.
Results: The PRP obtained by dual-spin centrifugation at 1500 rpm for 5-5 min showed the highest platelet recovery rate with no other white blood cell contamination. Consistently, platelets obtained from this PRP showed the maximum platelet aggregation in response to 2-MeSADP and thrombin, suggesting that optimizing the centrifugal speed and time is indispensable for the utmost collection of PRP with optimal platelet function. Interestingly, PDGF-BB release was dramatically increased time-dependently in PRP adjusted at 1 X 109 platelets/ml indicating the importance of platelet concentration in PRP for maximum GF release.
Conclusions: In conclusion, we standardized the PRP preparation with the optimal platelet function and GF release that will help to achieve the effectiveness and efficacy of the application of PRP in veterinary regenerative medicine.
To cite this abstract in AMA style:Kim S, Chaudhary PK, Kim S. Standardization of Platelet-rich Plasma Preparation with Optimal Platelet Function for the Use in Veterinary Regenerative Medicine [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/standardization-of-platelet-rich-plasma-preparation-with-optimal-platelet-function-for-the-use-in-veterinary-regenerative-medicine/. Accessed November 28, 2023.
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