Abstract Number: PB2519
Meeting: ISTH 2020 Congress
Background: Preeclampsia (PE) is a serious hypertensive and multi-system disease of pregnancy that has a challenging diagnosis. Neuroserpin (NS) is a protein involved in neurological, hemostatic and immune (e.g. Th17 cell response) processes that has not been evaluated in PE to date.
Aims: Investigate NS and interleukin (IL)-17 plasma levels and their association with hemostatic factors (PAI-1, D-dimer, von Willebrand factor, ADAMTS-13, FVII, FVIII) and clinical parameters of preeclamptic, normotensive pregnant and non-pregnant women.
Methods: All enrolled women signed an informed consent and the study was approved by the participant institutions. ELISA assays were performed for all the measurements in the plasma obtained from 25 non-pregnant, 26 normotensive pregnant and 21 PE women. These data were analyzed by ANOVA with post-hoc Tukey (parametric) or Kruskal-Wallis with post-hoc Dunn (nonparametric) tests. The correlations were analyzed by Spearman coefficients (Rs). Principal component analysis was performed combining clinical and laboratory data of the participants.
Results: NS plasma level was increased in normotensive pregnant women. However, its level in PE was comparable to the non-pregnant state (Figure 1A). Plasma IL-17 was up-regulated during pregnancy, but it was similar between normotensive and PE women (Figure 1B). IL-17 and NS were strongly and positively correlated when all the participants (Rs=0.820, P< 0.001) or only PE women (Rs=0.725, P< 0.001) were included in the analysis. In PE women, IL-17 and PAI-1 levels were strongly and negatively correlated (Rs=-0.714, P=0.047). Other weaker correlations were detected among NS, IL-17 and hemostatic factors. NS, IL-17 and FVII, combined with clinical parameters, were able to differentiate PE women (upper quadrants) from normotensive pregnant (lower quadrants) and non-pregnant (left lower quadrant) women (Figure 2).
Conclusions: NS and IL-17 may participate in PE pathogenesis and could integrate a new screening tool for PE diagnosis.
To cite this abstract in AMA style:Oliveira Perucci L, Pio Gomes da Silva S, Gonçalves de Moraes LÂ, Nessralla Alpoim P, Carvalho Godoi L, Sant'Ana Dusse LM, Talvani A. Systemic Evaluation of Neuroserpin, Interleukin-17 and Hemostatic Factors in Association with Clinical Parameters in Preeclamptic Women: A Principal Component Analysis Approach [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/systemic-evaluation-of-neuroserpin-interleukin-17-and-hemostatic-factors-in-association-with-clinical-parameters-in-preeclamptic-women-a-principal-component-analysis-approach/. Accessed November 28, 2021.
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