Abstract Number: PB1598
Meeting: ISTH 2020 Congress
Background: Recent work by our group revealed insufficient stromal support in JAK2V617F-positive ET resulting from molecular and functional deficits of the microenvironment. IFNα-2b has been used to treat ET patients, while the role of IFNα-2b on the microenvironment remains unclear.
Aims: This study aims to explore the functional and molecular effects of IFNα-2b on the microenvironment of ET.
Methods: We collected specimens from clinical patients, and then combined qPCR, western blotting, flow cytometry, immunochemiluminescence, liquid chip and so on to perform functional difference analysis of the microenvironment before and after IFNα-2b treatment. Then, based on the existing literature and database information, the molecular mechanism is explored, and the function of potential target molecules is verified according to Coho´s law.
Results: IFNα-2b had no effect on the morphology, immunophenotype and JAK2 mutant allele of BM-MSCs derived from ET patients. IFNα-2b was capable of suppressing the proliferation of BM-MSCs, but increasing its apoptosis and its function of supporting hematopoiesis. IFNα-2b also ameliorated its defective in inhibiting the proliferation of PBMCs and promotes PBMCs to differentiate into regulatory T cells. IFNα-2b affects the phosphorylation levels of multiple signaling pathways such as AKT and ERK, which may be the molecular mechanism by which it exerts biological effects.
Conclusions: IFNα-2b affects BM-MSCs from ET patients in multiple differentiation capacities, proliferation, apoptosis and the capacity to support long-term hematopoiesis and immune regulation. AKT and ERK signaling pathways may be the molecular mechanism by which it exerts biological effects.
To cite this abstract in AMA style:Sun T, Zhang A. Systmetic Study of Biological Properties and Functions of Bone Marrow Mesenchymal Stem Cells from Patients with Essential Thrombocythemia and Explore the Effects of Interferonα-2b on Them [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/systmetic-study-of-biological-properties-and-functions-of-bone-marrow-mesenchymal-stem-cells-from-patients-with-essential-thrombocythemia-and-explore-the-effects-of-interferon%ce%b1-2b-on-them/. Accessed January 28, 2022.
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