Abstract Number: PB1985
Meeting: ISTH 2020 Congress
Theme: Vascular Biology » Inflammation and Sepsis
Background: Neutrophils play an important antimicrobial role in the immune system. However, inappropriate neutrophil functions and hyperactivity lead to pathological inflammation and tissue damage.
Aims: This study investigates anti-inflammatory effect of the underlying mechanism of 3´-hydroxygenkwanin (ASSB-21) from Aquilaria sinensis in human neutrophils.
Methods: Signaling pathways regulated by ASSB-21 to oppose fMLP-induced respiratory burst, surface marker, migration were evaluated by immunoblotting analysis and flow cytometry.
Results: Neutrophils were activated with N-formyl-methionyl- leucyl-phenylalanine (fMLP) and phorbol 12-myristate 13 acetate (PMA) in this study. Briefly, ASSB-21 inhibited fMLP-induced reactive oxygen species (ROS) production (IC50: 6.03±1.03 µM), cathepsin G released (IC50: 27.51±6.80 µM) and neutrophil chemotaxis in a concentration dependent manner without affecting these induced by PMA. ASSB-21 (5-20 µM) inhibited fMLP-induced Akt phosphorylation. ASSB-21 (10-20 µM) inhibited fMLP-induced PIP3 levels indicated that ASSB-21 affected the activity of PI3K. In addition, ASSB-21 (20-50 µM) did not scavenge oxygen free radical in the xanthine/xanthine oxidase system. Theneutrophil viability and toxicity did not affect by ASSB-21 (20-50 µM). Furthermore, ASSB-21 (20 µM) inhibited PMA-induced neutrophil extracellular traps (NETs) formation. ASSB-21 (5-20 µM) inhibited PMA-induced Akt phosphorylation. However, ASSB-21 (20 µM) did not inhibit fMLP-induced and PMA-induced CD11b expression, intracellular calcium mobilization, phosphorylation of ERK1/2 and p38.
Conclusions: According to this data, ASSB-21 inhibited fMLP-induced neutrophils ROS production, cathepsin G released and chemotaxis by inhibiting PI3K pathway.
To cite this abstract in AMA style:
Liao HR, Chen J-, Wang S-. The Anti-Inflammatory Effects Of 3´-Hydroxygenkwanin from Aquilaria sinensis in Human Neutrophils [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/the-anti-inflammatory-effects-of-3-hydroxygenkwanin-from-aquilaria-sinensis-in-human-neutrophils/. Accessed March 21, 2024.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/the-anti-inflammatory-effects-of-3-hydroxygenkwanin-from-aquilaria-sinensis-in-human-neutrophils/