Abstract Number: PB0593
Meeting: ISTH 2020 Congress
Background: Light transmission aggregometry (LTA) is known as a gold standard method for assessing platelet function. The fully automated coagulation analyzer CS-5100 is already enabled to measure platelet aggregation using LTA. Recently, a new automated coagulation analyzer called CN-6000 was launched.
Aims: In this report, we have evaluated performance of the CN-6000 of platelet aggregation function with 2.0 µM adenosine diphosphate (ADP), 1.0 mM arachidonic acid, 2.0 µg/mL of collagen, 5.0 µM epinephrine and 1.2 mg/mL ristocetin.
Methods: We evaluated within-run precision, on-board stability and comparative study. Platelet-rich plasma (PRP) samples from healthy volunteers were used as normal samples and 1.0 mM of aspirin-spiked PRP or 0.05 µM of cangrelor (P2Y12 inhibitor)-spiked PRP were used as abnormal samples. Within-run precision was measured using 30 replicates of analysis. On-board stability was evaluated at 0, 4, 8 and 10 hour intervals. Comparative study between CN-6000 and CS-5100 was performed using 130 PRP samples with different levels of aggregation by adding several concentration of aspirin or cangrelor.
Results: For within-run precision evaluation, the coefficient of variation (CV) with all agonists tested with normal and abnormal samples was less than 5% and 13%, respectively. For on-board stability evaluation, the changes between 0 and 10 hours were within 5% CV, with each agonist.
In comparative study, y = 1.08x -1.34, r = 0.978 for ADP, y = 1.01x +1.62, r = 0.995 for arachidonic acid, y = 1.01x + 0.61, r = 0.989 for Collagen, y = 1.06x -1.61, r = 0.980 for epinephrine, y = 0.98x +5.28, r = 0.989 for ristocetin.
Conclusions: Our results demonstrated that the CN-6000 is a robust automated coagulation analyzer for platelet aggregation testing. The CN-6000 also provides high analytical performance as a high throughput analyzer for routine and specific coagulation parameters and is suitable for total laboratory automation.
To cite this abstract in AMA style:
Egashira M, Kono M, Nakajima K, Sakayori T. The Basic Evaluation of Light Transmission Platelet Aggregation Method on an Automated Coagulation Analyzer CN-6000 [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/the-basic-evaluation-of-light-transmission-platelet-aggregation-method-on-an-automated-coagulation-analyzer-cn-6000/. Accessed March 21, 2024.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/the-basic-evaluation-of-light-transmission-platelet-aggregation-method-on-an-automated-coagulation-analyzer-cn-6000/