Abstract Number: PB1709
Meeting: ISTH 2020 Congress
Theme: Platelets and Megakaryocytes » Platelet Function and Interactions
Background: Ceramic bone substituting materials are used for bone tissue repair. One of the most important characteristics of these materials is the size of pores in them. While interacting with implant platelets not only form osteoconductive matrix but also induce regeneration process.
Aims: To study aggregation activity of platelets after interacting with bone substituting materials with different physical and chemical properties.
Methods: We used Husky breed rats in the experiments. To get platelets-rich plasma (PRP) blood was centrifuged for 7 minutes with 1500 rmp. PRP was deluted to 360*1000/µL using platelets-poor plasma. As bone substituting materials we used: 1) Cа3(PO4)2 (b-TCP), material with open cell porous 10-50 µm, pore fraction ~35%; 2) La1.95Ca0.05Zr2O7, (lanthanum zirconate), close cell porous material, pore size 2-30 µm, pore fraction ~15%). Materials were granule-shaped, overall volume of each material was 80 mm3. Interaction between bone substituting materials and PRP conducted at 37°С, stirring for 30 minutes. Control samples underwent the same procedure but without bone substituting materials. Parameters of ADP-induced Bourne aggregation were measured. Final ADP concentration in samples was 20 µM. Nonparametric statistics were used. Kruskal-Wallis test and further multiple analysis were held. Research was approved by local ethics committee. All experiments were conducted in accordance with Helsinki Declaration.
Results: ADP induced the aggregation of platelets in all examined PRP samples. After interaction with lanthanum zirconate granules, the level of maximum platelet aggregation and the mean aggregate size did not exceed these indicators in the control group. After interacting with b-TCP granules maximum aggregation of platelets and mean size of aggregates were significantly larger then with lanthanum zirconate and control (table, picture).
Conclusions: Physical and chemical properties of bone substituting ceramic materials influence significantly on platelets activity, which can be of great importance in the process of osseointegration in the treatment of bone defects.
Control | b-TCP | Lanthanum zirconate | |
1 | 2 | 3 | |
Maximum platelet aggregation (%) | 26,53 [26,26; 27,06] | 33,29 [30,47; 35,78] | 27,45 [27,23; 27,93] |
P 2-1 <0,05 P 2-3 <0,05 | |||
Mean aggregate size (conventional units) | 7,73 [7,57; 7,76] | 13,95 [13,72; 14,06] | 10,86 [10,81; 11,08] |
P 2-1 <0,05 P 2-3 <0,05 |
[ADF-induced platelet aggregation after interacting with ceramic bone substituting materials. Results are presented as Med [IQR]]
[ADF-induced platelet aggregation. (A) Maximum platelet aggregation (%). (B) Mean aggregate size (conventional units).]
To cite this abstract in AMA style:
Nikanorova AD, Antropova IP, Gilev MV, Izmodenova MU, Khatsko SL, Petrova IM. The Effect of Ceramic Materials on Platelet Aggregation [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/the-effect-of-ceramic-materials-on-platelet-aggregation/. Accessed March 22, 2024.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/the-effect-of-ceramic-materials-on-platelet-aggregation/