Abstract Number: PB0524
Meeting: ISTH 2020 Congress
Background: Platelet count is measured by automated cell counters by various principles including impedance, optical and fluorescence. Variations have been noted between the different methodologies. We undertook this study to determine whether changes in RBC size or structure influence platelet count variation in patients with hemoglobinopathies.
Aims: 1. To determine the percentage difference between platelet values measured by impedance (PLT-I) and fluorescence-optical (PLT-F) in patients with Beta-Thalassemia, HbE and HbE/Beta-Thalassemia (HbE-BT). 2. To determine whether the calculated percentage difference between PLT-I and PLT-F showed any correlation with type of hemoglobinopathy, Micro-RBC% or FRC%.
Methods: 40 patients with either Beta-Thalassemia, HbE or HbE/B-T detected by high performance liquid chromatography in our hospital between April 2019 and January 2020 whose samples were run in both PLT-F and Reticulocyte Mode on the Sysmex XN 1000 were included in the study. PLT-F, PLT-I, RBC count, Mean Cell Volume (MCV), Micro RBC%, FRC% and Hemoglobin F% data was collected. Statistical analysis was performed.
Results: HbE/B-T showed the maximum percentage difference (27.81%) between PLT-F and PLT-I while HbE showed the least difference (8.12%). The mean HbF% was 4.85% and the mean FRC% was 1.45%. Statistically significant positive correlation was seen between PLT-I and PLT-F percentage difference and HbF as well FRC% (p< 0.05) by Pearson correlation test. The mean MCV was 64.5fl, while the mean Micro RBC% was 47.59%. No significant correlation was seen between PLT-I and PLT-F percentage difference and MCV, Micro RBC% or RBC count in these patients.
Conclusions: Significant correlation between PLT-I and PLT-F percentage difference and HbF and FRC% in patients with Beta-Thalassemia, HbE and HbE/Beta-Thalassemia suggest that RBC and hemoglobin structure may play a role in automated platelet measurement interference in hemoglobinopathies.
To cite this abstract in AMA style:
Shekhar R, Pai S, V S, Bhat V. The Effect of RBCs on Different Platelet Count Enumeration Methods in Patients with Hemoglobinopathies [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/the-effect-of-rbcs-on-different-platelet-count-enumeration-methods-in-patients-with-hemoglobinopathies/. Accessed March 21, 2024.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/the-effect-of-rbcs-on-different-platelet-count-enumeration-methods-in-patients-with-hemoglobinopathies/