Abstract Number: PB0765
Meeting: ISTH 2020 Congress
Background: Considering the continuing increase of morbidity and mortality rates associated with cardiovascular diseases, the search for novel compounds, which are able to affect the hemostasis system, are among the current trends of modern science and pharmacology.
Aims: To study the effects of fibrino(geno)lytic enzymes from the Antarctic hydrobionts (Odontaster validus, Sterechinus neumayeri) on hemostasis system.
Methods: The fibrino(geno)lytic enzymes were isolated by a complex approach which included affinity chromatography and size-exclusion chromatography. The influence of fibrino(geno)lytic enzymes on platelets aggregation was assessed using a aggregometer AT-02 (Medtech, RF). Fbrin(ogen)olytic activity was assessed by examination a cleavage pattern of the fibrinogen in SDS-PAGE after incubation with the enzymes. Activated partial thromboplastin time (aPTT), prothrombin time (PT), and thrombin time (TT) were measured using coagulation analyzer (Rayto, Shenzhen, China). The substrate specificity of enzymes was measured using chromogenic substrates H-D-Phe-Pip-Arg-pNA, pyroGlu-Pro-Arg-pNA, H-D-Val-Leu-Lys-pNA, and Bz-IIe-Glu(γ-OR)-Gly-Arg-pNA (Sigma, USA).
Results: The fibrino(geno)lytic enzyme from S. neumayeri cleaved preferentially the Aα-chain and more slowly the Bβ-chain of fibrinogen. The action of the enzyme from O. validus was similar, but lower. None of the proteases degraded the γ-chain of fibrinogen. Both fibrino(geno)lytic enzymes mediated the prolongation of clotting time in all chronometric tests. The degree of platelet aggregation was 20% and 31%, respectively after incubation with the fibrino(geno)lytic enzyme from S. neumayeri and O. validus. Enzymes also exhibit activity against chromogenic substrates more expressed in case of pyroGlu-Pro-Arg-pNA, which is a specific synthetic substrate for activated protein C and factor XIa.
Conclusions: In this study, we provide evidence that enzymes isolated from the tissues of Antarctic marine hydrobionts act as a fibrin(ogen)olytic enzymes. Further investigations of these enzymes are interesting as they can be of medical interest as therapeutic agents in the treatment and prevention of thrombotic disorders.
To cite this abstract in AMA style:Raksha N, Savchuk O, Ostapchenko L. The Effect of the Fibrino(geno)lytic Enzymes from Tissues of Antarctic Hydrobionts on Hemostasis [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/the-effect-of-the-fibrinogenolytic-enzymes-from-tissues-of-antarctic-hydrobionts-on-hemostasis/. Accessed March 3, 2021.
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