Abstract Number: PB0486
Meeting: ISTH 2020 Congress
Background: Blood collection systems have been designed to divert the first flow of blood into a diversion pouch (DP) that lacks anticoagulants. These systems help reduce blood contamination from skin pathogens. Blood collection is a critical preanalytical variable to control in preparation for functional testing of platelets. Having access to samples from healthy blood bank donors offers the possibility to study diverse aspects of platelet biology such as age, sex, or race from a vast number of individuals.
Aims: To determine if the functional profiles of platelets isolated from the diversion pouch (DP) as well as from apheresis (Aph) concentrates are representative of platelets isolated from venipuncture with immediate anticoagulation (VP).
Methods: Whole blood from the DP was collected into citrated tubes within two minutes of collection. Platelet-rich plasma (PRP) and washed platelets (WP) were isolated following standard protocols. For a subset of donors (N=6), platelets from the DP were also compared to Aph concentrates isolated on the same day and from the same individual. Baseline (resting) and activation (thrombin [0.1 IU/ml], ADP [5µM], and collagen [2 µg/ml]) profiles were determined by flow cytometry using anti-P-selectin and PAC-1 antibodies. Platelet aggregation was assessed by light-transmission aggregometry (LTA) of PRP using thrombin and collagen. Ongoing platelet metabolome analysis is pending.
Results: Platelets from blood isolated through standard VP, DP, and Aph exhibited no differences in P-selectin and PAC-1 signal at baseline. Similarly, all groups exhibit the same degree of activation by LTA and flow cytometry for all the agonists (Figure 1).
Conclusions: Platelets isolated from blood collected from the diversion pouch and apheresis exhibit a similar activation profile as platelets isolated per standard methods and are suitable options for functional testing analysis.
To cite this abstract in AMA style:Davizon-Castillo P, Kelly K, Kelher M, Manco-Johnson M, Silliman C, Dumont L. The Functional Profiles of Platelets Collected from Blood Diversion Pouches and Apheresis Concentrates Are Comparable to the Ones from Platelets Isolated through Standard Methods [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/the-functional-profiles-of-platelets-collected-from-blood-diversion-pouches-and-apheresis-concentrates-are-comparable-to-the-ones-from-platelets-isolated-through-standard-methods/. Accessed January 26, 2022.
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