Abstract Number: PB0547
Meeting: ISTH 2020 Congress
Background: The PFA-200 analyzer is used for screening of impaired primary haemostasis and for evaluation of antiplatelet drugs therapeutic effectiveness. The results are reported as closure time (CT) (the time to occlusion of the aperture). The blood volume (BV) (the volume of blood flowed through the aperture until its obstruction) is reported using the research mode. Three cartridges are available: COL/EPI with collagen and epinephrine as inductors, COL/ADP with collagen and ADP, and P2Y with ADP, prostaglandin E1 and calcium chloride. The results are affected by thrombocytopenia and anemia using COL/EPI and COL/ADP cartridges; this has not yet been reported with P2Y cartridge.
Aims: To evaluate the correlation of CT and BV results with platelet count (PLT), plateletcrit (PCT), immature platelet fraction (IPF), mean platelet volume (MPV), hematocrit (HCT) and product of PCT and HCT (PCTXHCT).
Methods: Patients with thrombocytopenia (PLT< 100x109/L) and no medication affecting platelet function were investigated using all three PFA cartridges. The correlation analysis was used for statistical analysis using the STATISTICA CZ software.
Results: 54 patients (30 males, 24 females, mean age 65 (range 19 to 93)) were investigated. The results of selected blood count values are summarized in table No 1.
No correlation of MPV with PFA results was found. The correlations of PFA results with others investigated blood count values are summarized in table No 2.
Conclusions: 1. The results of CT and BV are significantly affected not only by blood platelets count and PCT, but also by HCT; the role of HCT is at least as strong as the role of PLT and PCT. 2. Using the P2Y cartridge, the correlations of CT and BV with HCT are stronger than the correlations with PLT and PCT. 3. Using the P2Y cartridge, the correlation of CT and BV with IPF is significant.
|PLT (*10^9/L)||MPV (fL)||PCT (%)||IPF (%)||HCT|
[Table No 1: The results of selected blood count values:]
|COL/ADP CT||COL/ADP BV||COL/EPI CT||COL/EPI BV||P2Y CT||P2Y BV|
|PLT||r=-0.3713 p=0.011||r=-0.4976 p=0.000||r=-0.3279 p=0.026||r=-0.3977 p=0.006||r=-0.1883 p=0.210||r=-0.1879 p=0.211|
|PCT||r=-0.2950 p=0.047||r=-0.4436 p=0.002||r=-0.3521 p=0.016||r=-0.3696 p=0.011||r=-0.1096 p=0.468||r=-0.1094 p=0.469|
|IPF||r=-0.3301 p=0.025||r=-0.2852 p=0.055||r=-0.0909 p=0.548||r=-0.2777 p=0.062||r=-0.5939 p=0.000||r=-0.6523 p=0.000|
|HCT||r=-0.4500 p=0.002||r=-0.5416 p=0.000||r=-0.3413 p=0.020||r=-0.5959 p=0.000||r=-0.4623 p=0.001||r=-0.5703 p=0.000|
|HCTxPCT||r=-0.4556 p=0.001||r=-0.5840 p=0.000||r=-0.4438 p=0.002||r=-0.5443 p=0.000||r=-0.2738 p=0.066||r=-0.3086 p=0.037|
[Table No 2: The correlations of PFA results and selected blood count values. r correlation coefficient p statistical significance]
To cite this abstract in AMA style:Kessler P, Smejkalova M, Bradacova P, Prokopova J, Vagnerova J, Ullrychova J, Poul H, Harudova M, Vaskova A, Kohoutek T. The PFA Results Are Affected by Low Number of Platelets, Low Hematocrit and High Proportion of Immature Platelets [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/the-pfa-results-are-affected-by-low-number-of-platelets-low-hematocrit-and-high-proportion-of-immature-platelets/. Accessed October 2, 2023.
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