Background: The healthy endothelial cells are essential for maintenance of vascular homeostasis involving antioxidant, anti- inflammatory, pro- fibrinolytic, anti- adhesive or anticoagulant effects. As is well recognized, the majority of atherosclerosis start with a vascular injury, resulting in endothelial dysfunction.

Aims: In this study, we aimed to investigate the effect of the red laser- mediated photobiomodulation therapy, accompanied by aspirin administration on endothelial function in the rabbit model of carotid artery injury.

Methods: Briefly, New Zealand white rabbits underwent balloon dilatation injury at the right common carotid arteries. After 2 days, common carotid arteries of the treatment group at the lesion region, treated using extracorporeal low- level red laser (ƛ= 635 nm, E=100 J/cm2)- mediated photobiomodulation therapy accompanied by aspirin (10 mg/Kg) administration. In order to evaluate endothelial- dependent relaxation, acetylcholine- mediated dilation (AMD) was measured during the infusion of acetylcholine at a rate of 0.5 g/kg/ min and endothelial independent relaxation was evaluated by measuring nitroglycerin mediated dilation (NMD) during the infusion of nitroglycerin at a rate of 5 g/kg/min.

Results: Results from B- mode ultrasound images showed significant differences in AMD between the treated and the non- treated rabbits (P>0.05), whereas there were no significant differences in NMD between the treatment and normal groups (P>0.05). No microscopic intimal lesions were seen in the normal and treated rabbits, but intimal thickening was observed in the histological studies in the non- treated rabbits.

Conclusions: Low- level red laser- mediated photobiomodulation therapy accompanied by aspirin administration can cause to the improve endothelial dysfunction via increasing endothelial nitric oxide (NO) synthase. This protocol may be a potential treatment to endothelial dysfunction after balloon angioplasty.

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ISTH Congress Abstracts - https://abstracts.isth.org/abstract/the-red-laser-mediated-photobiomodulation-therapy-accompanied-by-aspirin-administration-improve-endothelial-dysfunction-after-balloon-angioplasty-in-the-rabbit-model-of-carotid-artery-injury/