Abstract Number: PB0039
Meeting: ISTH 2020 Congress
Theme: Arterial Thromboembolism » Atherosclerosis
Background: The endoplasmic reticulum (ER) is an important organelle involved in protein synthesis and folding, calcium homeostasis, and lipid biosynthesis. Recent findings indicate that atherosclerosis may be associated with impaired function of the ER. The ER resident protein 44 (ERp44) is a member of the protein disulfide isomerase family, which is induced by ER stress. However, the physiological role of ERp44 in atherosclerosis is still unclear.
Aims: To study the role of ERp44 in the atherosclerosis processes.
Methods: Thirty C57BL/6 mice and 90 adult ApoE-/- C57BL/6 mice were used as animal model in immunohistochemistry, real-time PCR and western blot (WB). HUVEC, THP-1 and HASMC were used as in vitro cell models in apoptosis, angiogenesis and migration assay.
Results: Immunohistochemical staining showed that a large amount of ERp44 expression in the aortic root of ApoE-/- high-fat diet mice at 6 weeks (Fig1A). Similar results were detected by PCR and WB (Fig1BC).
Apoptosis induced by ox-LDL was inhibited in HUVECs with high expression of ERp44 significantly. It showed that ERp44 inhibited the apoptosis by inhibiting Bax and p53 and promoting the expression of Bcl-2 (Fig2A).
It showed that angiogenesis inhibited by ox-LDL could be partially rescued by ERp44 through promoting the expression of PDGFRβ, VEGFR2 and EGFR (Fig2B).
It showed that over-expression of ERp44 in THP-1 could reduce the number of foam cells by promoting expression of cholesterol reverse proteins (ABCA1 and ABCG1) (Fig2C).
The increasing expression of cdc42 and AAMP might be the main reason for the enhancement of THP-1migration by ERp44 (Fig2D).
TUNEL staining showed over-expression of ERp44 significantly reduced the apoptosis of HASMC induced by ox-LDL, which was achieved by inhibiting Bax and p53, and promoting the expression of Bcl-2.
Conclusions: We found that ERp44 played an active role in the early and middle stages of atherosclerosis formation.
[Fig1. ERp44 expression in the aortic root of ApoE-/- high-fat diet mice]
[Fig2. In vitro cell assays]
To cite this abstract in AMA style:
Lu Q. The Role of ERp44 in the Atherosclerosis [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/the-role-of-erp44-in-the-atherosclerosis/. Accessed September 27, 2023.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/the-role-of-erp44-in-the-atherosclerosis/