Abstract Number: PB1683
Meeting: ISTH 2020 Congress
Background: The processes of primary hemostasis, which are caused by adhesion, aggregation and subsequent retraction of the platelet plug, cannot be considered in isolation from the processes of coagulation hemostasis. The final manifestation of both coagulation pathways is the appearance of fibrin filaments and the organization of a dense fibrin clot due to a reduction in its platelet component.
Aims: Investigate the role of fibrin in the interaction of lymphocytes and platelets isolated from blood.
Methods: The blood of practically healthy donors, with an adhesive surface in static conditions, we used fibrin-coated millipore filters. Served as control filters, impregnated with Na CL 0.9% (control 1) or autologous platelet-poor plasma (control 2). Pre-prepared filters were placed on the bottom of the wells of a plastic plate and pre-prepared lymphocyte-platelet suspension isolated from the blood of practically healthy individuals was placed. After incubation under static conditions and subsequent washing, the cell pool was resuspended and the number of lymphocyte-platelet coaggregates remaining in suspension was counted in a light microscope.
Results: It was established that after incubation of platelets and lymphocytes on the surface of fibrin filters, their amount in suspension was reduced almost 2.5 times compared to filters impregnated with NaCL 0.9% or platelet-poor plasma. At the same time, both the average number of platelets per lymphocyte and the total number of lymphocytes decreased (p < 0.05).
Conclusions: The decrease in their number in the suspension indicates the adhesion of lymphocytes in the composition of coaggregates with platelets to the fibrin coating.
Thus, lymphocytes are able to contact with fibrin, being in the composition of lymphocyte-platelet coaggregates аnd the presence of fibrin itself, and not of plasma, is of primary importance.
To cite this abstract in AMA style:Solpov A, Bogomyakova E, Solpova O, Vitkovsky Y. The Role of Platelets in the Adhesion of Normal Freshly Isolated Lymphocytes to Fibrin [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/the-role-of-platelets-in-the-adhesion-of-normal-freshly-isolated-lymphocytes-to-fibrin/. Accessed September 24, 2023.
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