Abstract Number: PB0272
Meeting: ISTH 2022 Congress
Background: Patients with hemolytic anemias are characterized by the increased risk for thrombosis. More and more studies have reported about procoagulant microparticles derived from the cells during hemolysis.
Aims: The aim of this study was to assess the contribution of procoagulant microparticles to the hypercoagulation in patients with hemolytic anemias.
Methods: The hemostasis of 5 children (boys, age: 4-12 yrs, girl 5 yr) with hereditary spherocytosis (HS) and 8 children with β-thalassemia major(β-TM) (2 girls 8 and 17 yrs, and 6 boys 2-15 yrs) was described using clotting times (APTT, PT), fibrinogen and D-dimer levels and thrombodynamics (TD) global hemostasis assay. The count of microparticles was assessed by flow cytometry. Five healthy donors were enrolled as a control group.
Results: Clotting times were normal in all patients but TD clot growth rate was increased in 7 children compared to normal range (59±2 µm/min for TD initial clot growth rate with normal range 38-56 µm/min). All patients with hypercoagulation were in severe condition: 3 patients with β-TM have no remission after splenectomy. The patients with high clot growth rates have the highest concentrations of phosphatidylserine-exposing microparticles(PS+MP) identified as procoagulant (1747±631 PS+MP/μl with normal range 306-606 PS+MP/μl). The patients without hypercoagulation have nearly 2 times lower counts of the procoagulant microparticles (740±378 PS+MP/μl). Glycophorin-exposing(CD235a+MP) microparticles were identified as erythrocyte-derived. Erythrocyte-derived microparticles were the highest in patients with the changes in the spatial dynamics of clot growth: an increase in stationary clot growth rate measured after fibrin layer was closed the activator surface with tissue factor.
Conclusion(s): Hypercoagulation was observed in patients with hemolysis in severe condition. The highest counts of procoagulant microparticles were in patients with hypercoagulation by clot growth rates. The highest counts erythrocyte-derived microparticles were revealed in patients with the disturbance of spatial dynamics of clot growth.
To cite this abstract in AMA style:Seregina E, Lipets E, Poletaev A, Ignatova A, Chuiko Y, Vuimo T, Ataullakhanov F, Smetanina N. The role of procoagulant microparticles in hemostasis of patients with hemolysis [abstract]. https://abstracts.isth.org/abstract/the-role-of-procoagulant-microparticles-in-hemostasis-of-patients-with-hemolysis/. Accessed September 22, 2023.
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