Abstract Number: PB1043
Meeting: ISTH 2021 Congress
Background: TGFβ1 and fibrinogen (F) are some of the key regulators of endothelial cells (EC) interactions in ALL which can modify the EC properties increasing their adhesiveness to neoplastics cells and their distribution in the body.
Aims: To determine the attachment of labeled by 3H-thymidine cells K562, L1210 to the monolayer of HUVECs in vitro after influence of plasma and conditional mediums (CM) off mononuclear cells from peripheral blood (MNPB) in 21 ALL pts who had not treated before investigation, and to compare obtained data with F concentration (FC) and endogenous TGFβ1 (enTGFβ1) level.
Methods: EnTGFβ1 level was detected by bioassay used CCL64 as a target. The MNPB were obtained after 24 h-incubation in RPMI 1640. The number of blasts in peripheral blood of ALL pts were 58,62±10,53%. The attachment of K562, L1210 to HUVECs were estimated by the index of adhesion (IA). The FC was estimated in venous citrate blood by simple hemostatic method.
Results: EnTGFβ1 concentrations in plasma and CM of MNPB of ALL pts were higher, than in plasma of donors (2,11±0,38 ng/ml; p<0,05). Elevated FC was found in ALL pts (7,20±0,60 mg/ml), than in donors (4,00±1,00; p<0,02). Plasma ALL pts showed comparatively strong influence on adhesive activity of HUVECs to K562 (IA=2,37±0,24), in comparison to L1210 (IA=1,28±0,8; p<0,05). CM of MNPB of ALL pts also intensively activated of adhesiveness of HUVECs (IA=1,99±0,38 for K562 and 1,92±0,24 for L1210, respectively). CM secreted higher level of TGFβ1, than plasma pts (p<0,05).
Conclusions: EnTGFβ1 and FC were elevated in plasma and CM of PBMC of ALL pts and may influence on spread of the ALL by stimulating of adhesiveness of endothelial cells. Therefore, the adjustment of enTGFβ1 and FC levels in the blood of ALL pts may be an important step in the treatment of AL.
To cite this abstract in AMA style:Barilka V, Matlan V, Prymak S, Shalay O. The Role of Transforming Growth Factor ß1 (TGFß1) and Fibrinogen in the Induction of Adhesive Properties of Vascular Endothelium (HUVECS) and the Spread of Acute Lymphoblastic Leukemia (ALL) [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/the-role-of-transforming-growth-factor-s1-tgfs1-and-fibrinogen-in-the-induction-of-adhesive-properties-of-vascular-endothelium-huvecs-and-the-spread-of-acute-lymphoblastic-leukemia-all/. Accessed November 29, 2021.
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