Abstract Number: PB1975
Meeting: ISTH 2020 Congress
Background: The endothelium serves an integral role in the hemostatic system. Endothelial cells are adapt their phenotype according to nature of local environment. Various component of thrombus can cause damage to endothelium in tissue culture and local environment can create deficient conditions. Since endothelial cells are important component of blood vessels, they can be triggered to induce angiogenesis upon stimulation.
Aims: The aim of the present work was to study biological effects of VEGF on endothelial cells in the conditions of unfed culture.
Methods: We used cultured aortic endothelial cell line (MAEC). Cells were incubated in condition of unfed culture. Cytotoxic/proliferative effect was determined using cytofluorymetric analysis. The level of glucose and NO in incubation medium were determined using a standard set.
Results: VEGF stimulates cell proliferation and increases number of cells in 1.6 and 2 times on the fifth day compared with control and anti-VEGF, respectively. The number of dead cells is the lowest under of influence VEGF. VEGF increased glucose consumption by cells 1.5 and 1.4 times compared with control and anti-VEGF, respectively, on the first day of incubation. However, on other days the number of cells was increased significantly, while consumption of glucose by cells was reduced. VEGF stimulates NO-production 2.3 times on the first day and 3.5 times on the second day compared with the control. Activating effect of anti-VEGF relative to the production of nitric oxide on the 4th-5th day of incubation is shown. Nitric oxide demonstrated multidirectional action, because the increase of its concentration under the influence of VEGF significantly stimulated the proliferation of endothelial cells, while its increase under the influence of anti-VEGF leads to increasing the number of apoptotic cells.
Conclusions: This study detected a relationship of proliferation, cell survival, glucose uptake and production of nitric oxide by endothelial cells in conditions of unfed culture.
To cite this abstract in AMA style:
Nikolaienko T, Garmanchuk L, Nikulina V. The survival of endothelial cells in the conditions of nutrient deficiency [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/the-survival-of-endothelial-cells-in-the-conditions-of-nutrient-deficiency/. Accessed March 22, 2024.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/the-survival-of-endothelial-cells-in-the-conditions-of-nutrient-deficiency/