Abstract Number: PB0128
Meeting: ISTH 2021 Congress
Background: Fibrin structure plays a crucial role in pathological events (Ząbczyk M, Undas A. Pol Arch Intern Med. 2017.).
Aims: To analyse the rheological properties of Fibrin clots formed in vitro in plasma from patients with carcinomatosis.
Methods: Fibrin clots formed in blood from peritoneal carcinomatosis patients (n=44) of different origins (colic:9, pseudomysoma:7, gastric:6, rectal:5, ovarian:6, others:11), from controls without cancer (n=31[CPG1] ) and from healthy volunteers (n=21) were analyzed. The viscoelastic parameters: clot elasticity or stiffness (G’) and clot viscosity (G”) were determined using an Anton-Paar Rheometer (oscillation, frequency 1 Hz, Strain (γ) 1%, time 1500 sec). G’ and G” determination was done using whole blood or fresh plasma. Fibrin clot degradation was performed using tissue plasminogen activator (tPA) incorporated into the clots and fibrin degradation products were evaluated using level of D-Dimers. The nucleotide materials in the clots were identified by confocal microscopy using DAPI and picogreen. Electron microscopy (SEM) analysis was performed using a S260 CAMBRIDGE apparatus to compare the structural shape and thickness of fibrin fibers in the clots.
Results: Our results indicated that:
i) In carcinomatosis patients, G’ is increased in 57% of patients,
ii) Ratio of G’/fibrinogen level is higher in cancer patients, as compared to controls,
iii) The plasma clot from cancer patients with high G’ value has low fibrin degradability (R = -0.55) compared to controls,
iv) Nucleotide material was detected at higher concentration in the clots from cancer patients compared to controls,
v) SEM analysis indicate a significant difference of morphological aspect as well as the thickness of fibrin fibers between Fibrin clots with low and those with high G’.
Conclusions: In cancer patients, the increase in visco-elasticity[CPG1] associated to an increased thickness[CPG2] of Fibrin fibers could explain the residual fibrin deposit on the peritoneal surface, probably due to a decreased fibrin degradation of clots.
To cite this abstract in AMA style:Ullah M, Mirshahi S, Aldybiat I, Contant G, Pocard M, Soria J, Mirshahi M. The Thickness of Fibrin Fibers is Associated with High Fibrin Clot Visco-elasticity in Carcinomatosis Patients [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/the-thickness-of-fibrin-fibers-is-associated-with-high-fibrin-clot-visco-elasticity-in-carcinomatosis-patients/. Accessed November 29, 2023.
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