Abstract Number: PB1007
Meeting: ISTH 2021 Congress
Theme: Platelets and Megakaryocytes » Platelet Function and Interactions
Background: Non-maturation of the arteriovenous fistula (AVF) leads to prolonged use of the central venous catheter (CVC) and can promote the thrombus development.
Aims: To evaluate the thrombogenic potential in patients undergoing hemodialysis with prolonged use of hemodialysis CVC and AVF.
Methods: This cross-sectional study included 68 adult participants with chronic renal failure. Participants were distributed: I: clinical and laboratorial healthy individuals matched by sex and age (controls); II: patients after one month of insertion of the catheter and AVF and III: patients after 4 months of insertion of the catheter and AVF. To the activation of platelets was investigated by GPIIb/IIIa and p-selectin expressions using flow cytometry. Thrombomodulin Elisa kit, and all tests were performed as recommended by the respective manufacturers and were run in duplicates.Statistical analysis was performed using the Student t, Mann-Whitney and ANOVA as appropriate. Differences statistically significant (p≤0.05). The participants signed the Informed Consent Term approved by the Ethics Committee, number 2,740,297.
Results: A significant difference was found by the expression of p-selectin comparing control group (32.84 ± 4.805) and the percentage obtained from peripheral vein at a time of 1 month (41.28 ± 5.849) and 4 months (50.08 ± 7.415) after CVC insertion (p <0.0001) (Figure 1).The median values for GPIIb/III expression were 10422(10033-10670),13933(13508-15509) and 19858 (17517-20712) after CVC insertion (p <0.0001), for groups I, II and III, respectively. To the AVF was not showed significant (p>0,05) changes in the platelet activation among groups I, II and III. The median values for Elisa test were 3547(3222-5057) for the patients after 4 months of the catheter and 2144(1754-3421) for the patients after 4 months of AVF.
Figure 1: Analysis of platelet activation by flow citometry. P-selectin analysis in percentages. Student’s test.
Conclusions: The current study demonstrated that the patients who use catheters for hemodialysis have a greater tendency to develop thrombi as adverse events when compared to those use AVF.
To cite this abstract in AMA style:
Dumont Cunha M, Henrique Fernandes Ottoni M, Araújo Figueiredo M, Coaglio Lucas T. Thrombogenic Potential in Patients Undergoing Hemodialysis Using Central Venous Catheter and Arteriovenous Fistula [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/thrombogenic-potential-in-patients-undergoing-hemodialysis-using-central-venous-catheter-and-arteriovenous-fistula/. Accessed November 29, 2023.« Back to ISTH 2021 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/thrombogenic-potential-in-patients-undergoing-hemodialysis-using-central-venous-catheter-and-arteriovenous-fistula/