Abstract Number: PB0422
Meeting: ISTH 2021 Congress
Theme: Fibrinogen, Fibrinolysis and Proteolysis » Fibrinolytic Factors and Inhibitors
Background: This research started when I learned that shochu lees (jiuqu) is optimal for enhancing the production effect of nattokinase, which is a thrombolytic enzyme.
Aims: The properties of this excellent novel thrombolytic enzyme will be described below. Koji-Natto (Kojikinase) showed not only a strong thrombolysis, vitamin K2 and polyamine contents, but also strong anti-cancer activity in HeLa cell culture system.
Methods: Thrombolytic activity, hydrolyzing for amide substrate, analysis of vitamin K2 and polyamine by HPLC were performed as described previously.
The anti-cancer activity was measured by culturing human HeLa cells and the necrosis efficiency of the cells. The number of cells was measured at an absorbance of 660nm.
Results: The fermented product was yellow to white, had little stickiness and odor, and was dried at 50oC to obtain a clean solid product.
It was found that the amidase activity is stronger against synthetic substrate I than synthetic substrate II (Sub I/Sub II=2.04), and that nattokinase also reacts with anti-nattokinase serum.
Kojikinase had an activity of 110IU/100g dry weight. It was also found that a considerable amount of vitamin K2 (800µg/100g) and polyamine (4,300µg/100g) were accumulated in the fermented product.
It was confirmed that when Kojikinase was added to the HeLa cell culture system, it strongly suppressed thrombolytic activity and completely necrotized HeLa cells at about 5mg/ml.
Conclusions: This method is characterized by not using soybeans at all. Therefore, according to the definition of natto, Koji-Natto is not natto. Jiuqu has been a technique for saccharification that can be dusty in Japan since ancient times, and has been widely used for making sake and miso. This method is a method of producing an extremely rare physiologically active substance using it. In particular, the strong inhibitory effect of Kojikinase on HeLa cells will be an important issue in the future.
To cite this abstract in AMA style:
Sumi H, Mitsuo T, Yatagai C. Thrombolysis and Anti-cancer Activity of Koji-Natto (Kojikinase) [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/thrombolysis-and-anti-cancer-activity-of-koji-natto-kojikinase/. Accessed November 29, 2021.« Back to ISTH 2021 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/thrombolysis-and-anti-cancer-activity-of-koji-natto-kojikinase/