Abstract Number: PB0424
Meeting: ISTH 2021 Congress
Background: Tempeh is a fermented soybean food produced by Rhizopus oligosporus, but it is known to have weaker thrombolytic activity than natto produced by Bacillus subtilis natto. However, tempeh is easier to eat because it has no odor or stickiness compared to natto, which is the same fermented food.
Aims: In this study, we report that we were able to obtain pearl-like tempeh with high thrombolytic activity by shaking culture of tempeh with a high-concentration substrate.
Methods: Rhizopus oligosporus purchased from Akita Konno Co., Ltd. was used, and standing culture was carried out as usual. In addition, some attempted shaking culture of the substrate at a high concentration. That is, 200ml of polypeptone S was used as a substrate in a 500ml Erlenmeyer flask in a shaking incubator (Yamato IM41), and the cells were cultured at 37oC for 10 days at 100rpm.
Results: As a result of shaking culture, the color of the medium changed from yellow to black at a substrate concentration of 2.5 to 60%, and in each case, a clean yellow pearl-like tempeh with a diameter of 0.2 to 1.0cm was obtained. The substance was durable and stable even when washed with water, and a clean dried product could be obtained by freeze-drying.
Thrombolytic activity peaked at 2.5% and 30-40% substrate concentrations. However, it was found that 70% or more of the thrombolytic activity was inactivated when this product was heat-dried at 50oC or higher.
Conclusions: Pearl-like tempeh could be obtained. The thrombolytic system of tempeh is fairly complex and may develop over time under more anaerobic constant conditions. This is because we have confirmed that tempeh bacteria significantly increase tyramine and cadaverine under more anaerobic conditions in petri dishes. New functional foods, especially the anti-cancer activity of pearl-like tempeh, are promising in the future.
To cite this abstract in AMA style:Sumi H, Yanagisawa Y, Naito S, Yatagai C. Thrombolytic Activity of Pearl-like Tempeh [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/thrombolytic-activity-of-pearl-like-tempeh/. Accessed August 16, 2022.
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