Abstract Number: PB1610
Meeting: ISTH 2020 Congress
Background: Platelets play an important role not only in hemostasis and thrombosis, but in many physiological or pathological processes including inflammation, immune response and fibrotic tissue formation. Therefore, investigation of thrombopoiesis is remaining to be one of the important problems in modern medicine. The source of thrombocytopoesis are matured megakaryocytes.
Aims: The aim of our study was investigation of megakaryocytes and observation of thrombocytopoesis in healthy persons, in patients with immune thrombocytopenia, acute myeloid leukemia, that sometimes is accompanied by thrombocytopenia, acute megacaryoblastic leukemia and primary myelophibrosis accompanying with prominent fibrosis.
Methods: For this aim we investigated bone marrow cultures of hematologically heathy donors and patients with abovementioned diseases. Thrombocytopoesis was observed in 3-36 – day bone marrow cell cultures. This culture method worked out by Shvelidze T. (1998, 2005) supports hemopoietic cells to maintain their proliferative activity and function.
Results: In patients with immune thrombocytopenia normal megakaryocytes containing mainly 6-7 fragmented nucleus with normal or increased platelet release were observed, in patients with acute myeloid leukemia megakaryocyte with endophagocytosis of myeloid blast cells was mentioned, in cases of primary myelofibrosis megakaryocytes contained 2-3 fragmented nucleus and non-active thrombocytopoesis was revealed. In the cases of acute megakaryoblastic leukemia abundance of magacaryoblasts, promegakaryocytes and misshaped megakaryocytes producing immature platelets was observed.
Conclusions: Results of our study shows that bone marrow cultures can be successfully used for the estimation of thrombocytopoiesis in norm and different pathological states. Besides thrombcytopoiesis following studies can be held to investigate platelet growth factors and other cytokines in vitro.
To cite this abstract in AMA style:Saralidze T, Svanidze T, Mamatsashvili I, Metskhvarishvili N, Rizhvadze M, Jangidze M, Noniashvili M. Thrombopoiesis in Bone Marrow Culture in Norm and in Some Pathological States [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/thrombopoiesis-in-bone-marrow-culture-in-norm-and-in-some-pathological-states/. Accessed January 28, 2022.
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