Abstract Number: PB2035
Meeting: ISTH 2020 Congress
Theme: Vascular Biology » Stem Cells and Vascular Cell Growth
Background: Bone marrow (BM) endothelial progenitor cells (EPCs) play a crucial role in regulating megakaryopoiesis. BM-EPCs are shown to be impaired in patients treated with chemotherapy. Our previous studies suggested that thrombopoietin (TPO) has a protective effect on hypoxia-induced apoptosis in human endothelial cells.
Aims: To explore whether TPO can protect BM-EPCs in chemotherapy patients and its underlying mechanism.
Methods: Firstly, CCK8 was used to explore the proliferative effect of BM-EPCs isolated from hematological patients treated with chemotherapy. Cells were divided into two groups: TPO-treated (experimental) group and TPO-free (control) group. BM-EPCs were identified by positively stained with CD34, CD309 and CD133. DiL-Ac-LDL uptake and FITC-UEA-I binding assay was performed to evaluate the amount of BM-EPCs; Secondly, the function of BM-EPCs in two groups were evaluated and compared by using tube-formation and migration experiments respectively. Lastly, BM-EPCs of two different groups were co-cultured with human megakaryocytes, and the proliferation of megakaryocytes was detected by flow cytometry.
Results: CCK8 results showed that TPO enhanced the proliferation of BM-EPCs as compared to the control group, and the optimal concentration is 100ng/ml. Flow cytometry indicated that TPO-treated cells had high expression of CD34/CD133/CD309. Double immunofluorescence assay indicated that the number of BM-EPCs in the experimental group was significantly higher than that in the control group (n=10; p=0.03); The second part data showed that the tube-formation
(n=10; p=0.02) and migration (n=10; p= 0.01) abilities of BM-EPCs in TPO-treated group were stronger than that of control group. The co-cultured experiment indicated that BM-EPCs in the experimental group significantly enhanced the human megakaryocytes proliferation（n=6; p=0.04).
Conclusions: Our data indicates that TPO may rescue megakaryopoiesis and reduce hemorrhage via protecting the function of BM-EPCs.
To cite this abstract in AMA style:
Zeng X, Jiao Y, Li Z, Zhang Y, Ye J. Thrombopoietin Rescues Megakaryopoiesis, Reduces Hemorrhage via Protecting Bone Marrow Endothelial Function of Chemotherapy-Treated Hematological Malignancies Patients [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/thrombopoietin-rescues-megakaryopoiesis-reduces-hemorrhage-via-protecting-bone-marrow-endothelial-function-of-chemotherapy-treated-hematological-malignancies-patients/. Accessed January 27, 2022.« Back to ISTH 2020 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/thrombopoietin-rescues-megakaryopoiesis-reduces-hemorrhage-via-protecting-bone-marrow-endothelial-function-of-chemotherapy-treated-hematological-malignancies-patients/