Abstract Number: VPB0618
Meeting: ISTH 2022 Congress
Theme: COVID and Coagulation » COVID and Coagulation, Basic Science
Background: SARS-CoV-2 tropism for the ACE2 receptor, along with the multifaceted inflammatory reaction, is likely to drive the generalized hypercoagulable state seen in patients with COVID-19.
Aims: We aimed identify microRNAs (miRNAs) play role in ACE2-related thrombosis in coronavirus infection and validated the expressions of those miRNAs in 79 hospitalized COVID-19 patients and 32 healthy volunteers and monitored miRNAs patterns during the acute phase of COVID-19, as well as the prognostic potential biomarker.
Methods: Using the original bioinformatic workflow and network medicine approaches we reanalyzed four coronavirus-related expression datasets and performed co-expression analysis focused on thrombosis and ACE2 related genes. Blood RNA; quality RNA was assessed: fluorometric assay; RT-PCR for miRNAs expression measurement.
Results: We identified EGFR, HSP90AA1, APP, TP53, PTEN, UBC, FN1, ELAVL1 and CALM1 as regulatory genes which could play a pivotal role in COVID-19 related thrombosis. We also found miR-16-5p, miR-27a-3p, Let-7b-5p and miR-155-5p as regulators in coagulation and thrombosis process. We observed in separate cohort of COVID-19 patients and healthy controls that (i) expression of miR-16-5p, miR-27a-3p and miR-155-5p increased during observation, compared to the baseline measurement; (ii) a low baseline miR-16-5p expression presents predictive utility in assessment of the hospital length of stay or death in follow-up as a composite endpoint (AUC:0.810, 95% CI, 0.71-0.91, p < 0.0001); (iii) low baseline expression of miR-16-5p and T2DM are independent predictors of increased length of stay or death according to a multivariate analysis (OR: 9.417; 95% CI, 2.647-33.506; p=0.0005 and OR: 6.257; 95% CI, 1.049-37.316; p=0.044).
Conclusion(s): This study enabled us to better characterize changes in gene expression and signaling pathways related to COVID-19 thrombosis. In this study we identified, characterized and validated miRNAs which could serve as novel, thrombosis-related biomarkers of the COVID-19, can be used for early stratification of patients and prediction of severity of infection development in an individual.
To cite this abstract in AMA style:
Eyileten C, Wicik Z, Simões S, Martins-Jr D, Keshwani D, Shahzadi A, Jarosz-Popek J, Wolska M, Klos K, Wlodarczyk W, Soldacki D, Chcialowski A, Siller-Matula J, Postula M. Thrombosis-related circulating miR-16-5p is associated with disease severity in patients hospitalised for COVID-19 [abstract]. https://abstracts.isth.org/abstract/thrombosis-related-circulating-mir-16-5p-is-associated-with-disease-severity-in-patients-hospitalised-for-covid-19/. Accessed March 22, 2024.« Back to ISTH 2022 Congress
ISTH Congress Abstracts - https://abstracts.isth.org/abstract/thrombosis-related-circulating-mir-16-5p-is-associated-with-disease-severity-in-patients-hospitalised-for-covid-19/