Background: For modern medicine and pharmacology, the most important are the problems of finding new drugs that are safe and without side effects. The use of drugs based on regulatory peptides can significantly help in solving these problems. However, until recently, few such peptides have been found.

Aims: To study the effect of small regulatory peptides, which are fragments of many natures of compounds, such as collagen, neurohormones, vasopressin, oxytocin, luliberin on platelet aggregation. Data on the effect of tripeptides on aggregation are presented on immobilization stress models and on the development of experimental inflammation.

Methods: Experiments were carried out on white mongrel male rats weighing 180-200 g body. A pro-inflammatory agent was used to simulate experimental peritonitis – 40% sodium thioglyaolate solution 4g / kg, administered intraperitoneally. We are using peptide Pro-Gly-Pro (PGP) fragment of collagen in dose 3,7 mkg/kg intramuscularly for prophylactic and treatment inflammation. Platelet aggregation (PA) was recorded on a aggregometer. Inductor aggregation – ADP -10 mM. In the next series of experiments, animals were subjected to immobilization stress for 60 minutes and determined the effect of C-terminal tripeptides of neurohypophysial hormones on PA. All experiments were performed according to the documents of the European Science Foundation.

Results: The development of inflammation causes an increase in PA with a maximum effect 2 hours after administration of thioglycolate. Prophylactic peptide administration significantly reduced PA, whereas peptide did not show any statistically significant results on the background of an already developed inflammation. C-terminal tripeptides of neurohypophysial hormones Pro-Arg-Gly and Pro-Leu-Gly also had a protective effect on PA against immobilization preventing the development of a prothrombotic stress effect.

Conclusions: Thus, we can note that the tripeptide of various origin can have a prophylactic protective effect, reducing PA in inflammation and immobilization stress.

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ISTH Congress Abstracts - https://abstracts.isth.org/abstract/tripeptides-affected-hemostas-in-various-pathologies/