Abstract Number: PO107
Meeting: ISTH 2021 Congress
Background: The laser atherectomy methods that are currently in use, cause to inflammation and subsequent restenosis.
Aims: The aim of this study was to evaluate the effect of combined 5- Aminolevulinic Acid- loaded microbubbles- mediated photodynamic therapy and catheter- based 192Ir- mediated β– brachytherapy on inflammation and intimal hyperplasia reduction after laser angioplasty of the animal occluded femoral artery, wherein diagnostic ultrasound is adjuncted with angioplasty and combination therapy system, with a goal of increased safety.
Methods: Briefly, New Zealand white rabbits were submitted to femoral artery advanced atherosclerotic occlusion by primary perivascular severe cold injury followed by a 2% cholesterol- rich diet for fourteen weeks. Histopathology results showed the formation of stable advanced atherosclerosis with lipid and neovessel – rich plaque, resulted in occlusion in all of the rabbits’ arteries. Then treatment group underwent B- mode ultrasound- guided argon laser (488 nm) angioplasty followed by catheter- based β– brachytherapy (192Ir, 15 Gy) in combination with photodynamic therapy with red diode laser (WL= 635 nm, E/A= 120 J/cm2) accompanied by photosensitizer 5- Aminolevulinic Acid- loaded PESDA (Perfluorocarbon- Exposed Sonicated Dextrose Albumin) microbubbles (100ml/kg, 2-5 ×105 bubbles/ml) administration and simultaneously ultrasound imaging.
Results: Results from ultrasonography and histopathology showed a significant reduction in the mean value for immune cells and smooth muscle hyperplasia cells density after angioplasty in the treatment group compared with the other groups (p<0.05).
Conclusions: Apoptotic effect of β– brachytherapy in combination with anti- inflammatory effect of 5- Aminolevulinic Acid, induced by photodynamic therapy effect of red diode laser, can cause to reduce the density of macrophage cells and smooth muscle hyperplasia cells in the intimal layer. These findings provide the basis for developing of combined β– brachytherapy and photodynamic therapy for a successful clinical application in the treatment of neointimal hyperplasia after laser angioplasty.
To cite this abstract in AMA style:Mehrad H, Ahmadi Noubari H. Ultrasound-guided Laser-mediated Thermal Angioplasty of Occluded Artery Accompanied by Combined 5-Aminolevulinic Acid-loaded Microbubbles-mediated Photodynamic Therapy and Catheter-based 192Ir-mediated β–brachytherapy [abstract]. Res Pract Thromb Haemost. 2021; 5 (Suppl 2). https://abstracts.isth.org/abstract/ultrasound-guided-laser-mediated-thermal-angioplasty-of-occluded-artery-accompanied-by-combined-5-aminolevulinic-acid-loaded-microbubbles-mediated-photodynamic-therapy-and-catheter-based-192ir-mediate/. Accessed December 7, 2021.
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ISTH Congress Abstracts - https://abstracts.isth.org/abstract/ultrasound-guided-laser-mediated-thermal-angioplasty-of-occluded-artery-accompanied-by-combined-5-aminolevulinic-acid-loaded-microbubbles-mediated-photodynamic-therapy-and-catheter-based-192ir-mediate/