Abstract Number: PB0650
Meeting: ISTH 2020 Congress
Background: Platelet count can be altered in various diseases and treatments and that may provide better insight into the expected outcome. So far, determination of platelet count is done within laboratory conditions by using established hematology analyzers, while a point-of-care device could be used for this purpose to speed up this process.
Aims: Our aim was to validate a newly developed point-of-care platelet counter device (PC100) by determining the bias between PC100 and a reference method (Sysmex XP-300).
Methods: Whole blood was taken from 60 healthy individuals into EDTA or ACDA. Basic platelet count in EDTA whole blood was measured by PC100 and compared to Sysmex XN-900LT hematology analyzer. Platelet rich plasma (PRP) was prepared from ACDA whole blood and platelet count was adjusted to the range 250- 3600 platelets (plts) /nL and measured with the PCI-100 and Sysmex XP-300. The PC100 platelet counter consists of a clinical analyzer and disposable glass slides, performing automatic enumeration of the platelets in two independent adjacent chambers (chamber A, B) providing two replicates as a result.
Results: Platelet count measured in whole blood correlated well between PC100 and the reference method. Platelet count determined in PRP by PC100 showed 1.42% median difference in comparison to the reference method. While the difference between two methods increased with concentration of platelets in PRP, a strong linear relationship remains throughout the whole interval (250- 3600 plts/nL) with high correlation coefficient (r=0.99). The slope obtained from linear regression analysis was 0.98 (95% CIs: 0.97-0.99), representing a 2% proportional difference between the two methods. Comparability assessment of the predicted bias at extreme concentrations of measuring interval, where the highest variations are expected, showed that the bias falls within the 10% acceptable range.
Conclusions: The PC100 platelet counter provides equivalent results compared to the reference method, Sysmex XP-300 hematology analyzer.
To cite this abstract in AMA style:Nagy M, van Oerle R, Fazaeli S, Sherry J, Giesen PLA, Lauwerijssen C, Mimnagh G, ten Cate H, Spronk HM. Validation of a New Point-of-Care Platelet Counter [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/validation-of-a-new-point-of-care-platelet-counter/. Accessed December 11, 2023.
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