ISTH Congress Abstracts

Official abstracts site for the ISTH Congress

MENU 
  • Home
  • Congress Archive
    • ISTH 2021 Congress
    • ISTH 2020 Congress
  • Resources
  • Search

Visualization of Thrombin Formation on Activated Platelets and Fibrin Formation in Venous Flow Condition

N. Sugita1, H. Hirakata2, K. Inoue2, K. Koike2

1Dept of Psychiatry, Kyoto University, Kyoto City, Japan, 2Dept of Emergency Medicine, Kyoto University, Kyoto City, Japan

Abstract Number: PB2374

Meeting: ISTH 2020 Congress

Theme: Venous Thromboembolism and Cardioembolism » VTE Prophylaxis

Background: Venous thromboembolism (VTE) is one of major causes of sudden death. Prevention of VTE is hard because of lacking understanding of mechanical and physical property of venous thrombus. Thrombin generation is most important phase for fibrin clot formation. Thrombin is explosively generated by prothrombinase complex on activated platelets.

Aims: Our aim of this study is visualization of time course of thrombin generation on activated platelets and fibrin formation in high magnification to elucidate extract physical property of venous thrombus generation at the point of view of fibrin formation in low shear stress.

Methods: Human venous blood was obtained from healthy volunteers. We separated platelets by centrifugation to stain with Alexa Fluor 488 and mixed them into the whole blood composition again. Alexa fluor 546-conjugated fibrinogen was added to visualize fibrin formation. BOC-Val-Pro-Arg-AMC (Boc-VPA), a sensitive fluorogenic substrate for alpha-thrombin, was added to visualize thrombin generation.
To meet our aim, we made a microfluidic device system to observe thrombus structure formed under venous shear rate conditions. The blood sample was introduced to a home build microfluidic channels that ware fully siliconized and partly coated with collagen. The thrombus image and thrombin were observed by a fluorescence microscope with 60X magnification.

Results:
1. Thrombin was generated at a same time explosively on every cluster of activated platelets.
2. Thrombin generation was accelerated shortly before formation of fibrin network.

Conclusions: Thrombin generation on activated platelets was visualized by a fluorescence microscope.

To cite this abstract in AMA style:

Sugita N, Hirakata H, Inoue K, Koike K. Visualization of Thrombin Formation on Activated Platelets and Fibrin Formation in Venous Flow Condition [abstract]. Res Pract Thromb Haemost. 2020; 4 (Suppl 1). https://abstracts.isth.org/abstract/visualization-of-thrombin-formation-on-activated-platelets-and-fibrin-formation-in-venous-flow-condition/. Accessed May 16, 2022.

« Back to ISTH 2020 Congress

ISTH Congress Abstracts - https://abstracts.isth.org/abstract/visualization-of-thrombin-formation-on-activated-platelets-and-fibrin-formation-in-venous-flow-condition/

Simple Search

Supported By:

Bristol Myers Squibb and Pfizer

ISTH 2021 Congress site

Visit the official web site for the ISTH 2021 Virtual CongressĀ Ā»

  • Help & Support
  • About Us
  • Cookies & Privacy
  • Wiley Job Network
  • Terms & Conditions
  • Advertisers & Agents
Copyright © 2022 John Wiley & Sons, Inc. All Rights Reserved.
Wiley