Abstract Number: PB1070
Meeting: ISTH 2022 Congress
Background: There is clinical interest in alternative diagnostic methodology for platelet function disorders in contrast to the time consuming and labour intensive “gold standard” of light transmission or lumiaggregometry.
Aims: Within this prospective study, we aimed to determine the sensitivity of whole blood impedance aggregometry (WBIA) with the Multiplate® analyser (Roche) in detecting platelet function abnormalities amongst women referred for surgical management of severe menorrhagia.
Methods: Women aged ≥18 referred for surgical management of menorrhagia at our tertiary gynaecology centre were eligible for inclusion. Anti-thrombotic therapy, known bleeding disorder or pelvic malignancy were exclusion criteria. After providing informed consent, participants underwent standardised ISTH-BAT scoring and laboratory testing, including platelet function testing with both lumiaggregometry and WBIA (Multiplate® analyser, Roche). Those with abnormal lumiaggregometry results underwent repeated testing by both methods and were also referred for specialist haematology review.
Results: Fifty patients were recruited and underwent testing. Eight women (16%) were identified as having a platelet function defect based on reproducible abnormalities on lumiaggregometry. Of these, 3/8 had results consistent with a storage pool disorder; medication was suspected to be a contributory factor in a further 3/8 cases. WBIA results were normal in 3/8 women (37.5%) in whom lumiaggregometry detected platelet dysfunction, 2 of whom had a storage pool disorder. Only 2 patients with a platelet function abnormality detected on lumiaggregometry had persistently abnormal WBIA analysis, giving a test sensitivity of only 25%. Five patients had abnormal WBIA results despite normal lumiaggregometry results.
Conclusion(s): In conclusion, WBIA with the Multiplate® analyser (Roche) has poor sensitivity and specificity in comparison to gold standard lumiaggregometry in the detection of platelet function disorders amongst women with severe menorrhagia referred for surgical intervention.
This study was supported by a grant from the Platelet Society and received local ethical approval.
To cite this abstract in AMA style:Delaney A, Maclean R, Makris M, Horner K, Kitchen S, Sedcole J, Baxter T, Samuelson C. Whole Blood Impedance Aggregometry Is Inferior to Lumiaggregometry in the Diagnosis of Platelet Function Defects Amongst Women With Menorrhagia Referred for Surgical Intervention [abstract]. https://abstracts.isth.org/abstract/whole-blood-impedance-aggregometry-is-inferior-to-lumiaggregometry-in-the-diagnosis-of-platelet-function-defects-amongst-women-with-menorrhagia-referred-for-surgical-intervention/. Accessed September 21, 2023.
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